Bio-Assay Guided Isolation of Germacranes with Anti-Protozoan <i>Activity from Magnolia sororum</i>

In the course of our search for antiprotozoal agents from terrestrial plants, three new germacranes 1, 2 and 3, together with the well known sesquiterpene lactones parthenolide (4) and costunolide (5), were isolated from Magnolia sororum using bioassay-guided fractionation methods. The structures of these new compounds were elucidated by 1D and 2D NMR spectroscopic analysis. Compound 5 exhibited activity (IC 50 = 9.4 μM) in vitro against the Leishmania mexicana parasite. Additionally, all compounds were also evaluated against Trypanosoma cruzi and Monkey Vero cells without showing much activity.

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